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Image Search Results
Journal: Nucleic Acids Research
Article Title: Phage display mediated immuno-PCR
doi: 10.1093/nar/gkl260
Figure Lengend Snippet: Detection sensitivity of indirect PD-IPCR and ELISA for NP. ( a ) Indirect PD-IPCR experiment. The polystyrene microtitre plate was coated with 100 µl of 10-fold serial dilutions of NP. Bound phages were used for PCR and amplification products were run on a 1.5% agarose gel. Lane 1 to 7 represent amplification products from 10 6 to 1 pg/ml. Lane 8 was negative control without NP coating. Lane M is DNA marker (DL2000) with 2000, 1000, 750, 500, 250 and 100 bp. ( b ) Sensitivity comparison between PD-IPCR and ELISA. The amplification band of PD-IPCR was quantified by a computer assisted image analyzer. The color development in ELISA was achieved with HRP-labeled anti-M13 monoclonal conjugate secondary antibody and TMB substrate. Average values of three independent experiments are given.
Article Snippet: Band density was determined by densitometry using a
Techniques: Enzyme-linked Immunosorbent Assay, Amplification, Agarose Gel Electrophoresis, Negative Control, Marker, Comparison, Labeling
Journal: Nucleic Acids Research
Article Title: Phage display mediated immuno-PCR
doi: 10.1093/nar/gkl260
Figure Lengend Snippet: Detection sensitivity of sandwich PD-IPCR and sandwich ELISA for NP. The amplification band of sandwich PD-IPCR was quantified by a computer assisted image analyzer. The color development in sandwich ELISA was achieved with HRP-labeled anti-M13 monoclonal conjugate secondary antibody and TMB substrate. Average values of three independent experiments are given.
Article Snippet: Band density was determined by densitometry using a
Techniques: Sandwich ELISA, Amplification, Labeling
Journal: Nucleic Acids Research
Article Title: Phage display mediated immuno-PCR
doi: 10.1093/nar/gkl260
Figure Lengend Snippet: Detection sensitivity of sandwich PD-IPCR and sandwich ELISA for PrP. The amplification band of PD-IPCR was quantified by a computer assisted image analyzer. The color development in ELISA was achieved with HRP-labeled anti-M13 monoclonal conjugate secondary antibody and TMB substrate. Average values of three independent experiments are given.
Article Snippet: Band density was determined by densitometry using a
Techniques: Sandwich ELISA, Amplification, Enzyme-linked Immunosorbent Assay, Labeling